DISC-3D: DUAL-HYDROGEL SYSTEM ENHANCES OPTICAL IMAGING AND ENABLES CORRELATIVE MASS SPECTROMETRY IMAGING OF INVADING MULTICELLULAR TUMOR SPHEROIDS

DISC-3D: dual-hydrogel system enhances optical imaging and enables correlative mass spectrometry imaging of invading multicellular tumor spheroids

Abstract Multicellular tumor spheroids embedded in collagen I matrices are common in vitro systems for the study of solid tumors that reflect the physiological environment and complexities of the in vivo environment.While collagen I environments are physiologically relevant and permissive of cell invasion, studying spheroids in such hydrogels prese

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More than meets the I(ris): Use of manual urine microscopy to complement automated findings in acute kidney injury

Evaluation of patients with acute kidney injury requires comprehensive assessment that includes a urinalysis, which features both semi-quantitative assessment with a urine dipstick and urine microscopy.This process is labor intensive for clinical laboratories, and availability of excellent welspun spaces double bedsheet automated instruments for ur

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Effect of supplementation with lutein, zeaxanthin, and omega-3 fatty acids on macular pigment and visual function in young adults with long-term use of digital devices: study protocol for a randomized double-blind placebo-controlled study

BackgroundGrowing evidence emphasizes the importance of xanthophyll carotenoids and omega-3 fatty acids in eye health.However, the beneficial effects of such supplementation have not been thoroughly discussed among adults with high screen exposure.Current trial evidence on lutein bioavailability is contradictory, and the interactions of dietary int

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Dielectrophoretic Traps for Efficient Bead and Cell Trapping and Formation of Aggregates of Controlled Size and Composition

We present a microfluidic dielectrophoretic-actuated system old fashioned cut rock candy designed to trap chosen single-cell and form controlled cell aggregates.A novel method is proposed to characterize the efficiency of the dielectrophoretic trapping, considering the flow speed but also the heat generated by the traps as limiting criteria in cell

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